AUGUSTUS

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AUGUSTUS is a gene prediction software package for eukaryotic genomes. It is a command-line application that uses probabilistic models (generalized hidden Markov models) and species-specific training to predict protein-coding genes, producing GFF/GTF-style annotations. AUGUSTUS can incorporate extrinsic evidence (hints) such as RNA‑seq alignments, ESTs, and protein homology to improve predictions.

The tool is widely used in genome annotation pipelines for de novo and comparative annotation, and...

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